Cell tracker dye. This probe overcomes the .

Cell tracker dye ; Synonyms: AIEDots Cell Tracker Dye, AIE Live Cell Fluorescent Dye, Fluorescent Cell Tracking Dye, Live Cell Fluorescent Nanoparticles at Sigma-Aldrich CellTracker Orange Dye Profile ThermoFisher Scientific™ 532. Supplier: Invitrogen™ C7025 View more versions of this product. From 11:00 pm to 12:00 pm EST ( 8:00 pm to 9:00 pm PST ) on January 6th, the website will be under maintenance. Cell aggregates are fixed in 4% paraformaldehyde/PBS for 30 min with gentle inversion, then washing cells in PBS for 3 times and stained with 100 ng/mL Hoechst dye overnight prior to imaging. 分子量: 209. Catalog No. The PKH dyes and, more recently, CellVue ® Claret 11 have served as fruitful probes for qualitative and quantitative Features of the CellTracker dyes include: Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells; Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations) ; Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion; Low cytotoxicity—does not affect viability or CellTracker Red CMTPX is a cell-permeable fluorescent dye that can be used as a cell tracer for monitoring cell movement and location (Ex/Em=586/614 nm). • Add 60 μL/vial of anhydrous DMSO to make a 1000X (1 mM) stock solution CellTracker CM-DiI is a DiI derivative that is somewhat more water-soluble than DiI, thus facilitating the preparation of staining solutions for cell suspensions and Invitrogen CellTracker CM-DiI Dye 20 x 50ug:Cell Analysis Products, Quantity: | Fisher Scientific Figure 1. The next day the cells (across three separate 96 well plates) were loaded with the indicated CellTracker at 1 µM concentration in Live Cell Imaging Solution (LCIS). 60 GBP valid until 2025-06-30 Use promo code "24074" to get your Features of the CellTracker dyes: • Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells • Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations) • Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion • Low cytotoxicity—does not affect viability or CellTracker dye는 세포 독성의 원인을 제공하지 않습니다. SubCellular Localization Actin, Cytoskeleton. . CellTracker® Orange CMRA is a fluorescent dye used for monitoring cell movement or location, allowing for multigenerational tracking of cellular movements. Hamburger Menu Button. $666. C7025. Here, we report a dual-activatable cell tracker for increased spatial and temporal staining control, especially for single-cell tracking. Fixed (aldehyde) Lysosomal staining: Cell-permeable dye that accumulates in the lysosome via the lysosome pH gradient (pH 4. 00 Specifications. 1 mg dry powder CellTracker™ Green CMFDA and CellTracker™ Orange CMTMR dyes 1 mg dry powder CellTracker™ Green CMFDA, CellTracker™ Orange CMRA, and CellTracker™ Red CMTPX dyes 20 × 50 µg dry powder CellTracker™ Deep Red dye 20 × 15 µg dry powder Approximate fluorescence Features of the CellTracker dyes include: Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells; Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations) ; Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion; Low cytotoxicity—does not affect viability or Features of the CellTracker dyes include: Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells; Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations) ; Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion; Low cytotoxicity—does not affect viability or Flow cytometry cell tracing probes are offered for short- and long-term cell tracing in fixable and non-fixable samples. Contains 1 vial with enough reagent to stain 120 slides, when 500 μL of 1X staining solution is used per slide. Sign in. Brand: Invitrogen™ C34552 320. - Mechanism of Action & Protocol. Cells loaded with the CellTrackerTM fluorescent probes display fluorescence for at least 72 hours and exhibit ideal tracking dye properties—they are stable, nontoxic at working concentrations Tag-it Violet™ Proliferation and Cell Tracking Dye can be used for cell tracking and for proliferation assays. CellTracker dyes do not contribute to cytotoxicity. Features of the CellTracker dyes include: Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells; Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations) ; Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion; Low cytotoxicity—does not affect viability or 赛默飞Invitrogen细胞示踪荧光探针是在复杂环境中追踪细胞的重要工具，广泛应用于细胞迁移、创伤愈合及干细胞分化研究。这些探针稳定无毒、可在细胞内稳定保留，并发出明亮荧光。多种颜色选择，方便与其他荧光探针共同使用。 Features of the CellTracker dyes include: Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells; Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations) ; Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion; Low cytotoxicity—does not affect viability or 产品描述. 04 GBP valid until 2025-04-18 Use promo code "24107" to get your Phalloidin-conjugated dye that binds to actin filaments but not to actin monomers or dimers. Applications of dye dilution proliferation analysis (Figure 1) have also grown dramatically in the last decade. Emission Max. CellTracker™ is a fluorescent dye well suited for monitoring cell movement or location. HeLa 세포는 웰당 2,500개 세포 밀도로 96-웰 플레이트에 플레이팅되었습니다. Cell-Tracker荧光探针是用来监测细胞运动、定位、增殖、迁移、趋化和侵袭的优秀工具。 Cell-Tracker荧光探针能自由穿透细胞膜进入细胞，在胞内转化生成不具细胞膜渗透性的反应产物。 Resuspended cells are stained with CellTracker Red CMTPX and allowed to self-assemble into spheroids before aggregation. Live: Mitochondrial staining: Cell-permeable dye that accumulates in the mitochondria via the mitochondrial membrane Cell trackers are fluorescent chemical tools that facilitate imaging and tracking cells within live organisms. HeLa cells were plated in 96-well plates at a density of 2,500 cells per well. Features of the CellTracker dyes include: Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells; Excellent retention—fluorescent signal retention of >72 hours (typically three to six Features of the CellTracker dyes include: Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells; Excellent retention—fluorescent signal retention of >72 hours (typically three to six Cells need to be viewed in less than 24 - 48 hours as dye will be passed to daughter cells and not to adjacent cells in a population. CellTracker色素は細胞に毒性はありません。 HeLa細胞を、1ウェルあたり2,500細胞の密度で96ウェルプレートに播種しました。 翌日、細胞に（3枚別々の96ウェルプレート） Live Cell Imaging Solution （LCIS）中 Cell-Tracker Green CMFDA；CellTracker™ Orange CMTMR；Cell movement 细胞运动；Cell location 细胞定位；Cell Tracker dye 细胞示踪探针；CAS NO：323192-14-9； 使用产品：Cell-Tracker Orange CMTMR and Cell Features of the CellTracker dyes include: Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells; Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations) ; Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion; Low cytotoxicity—does not affect viability or CMFDA，英文全称5-chloromethylfluorescein diacetate，是一种可自由透过活细胞膜对细胞进行示踪的荧光染料。 CMFDA是二乙酸荧光素（Fluorescein diacetate，FDA）的氯甲基衍生物，具细胞膜渗透性，不具有荧光发光性。 CellTracker™ Violet BMQC dye 5 × 0. 8). Tag-it Violet™ passively diffuses into the cell where esterases cleave acetoxymethyl esters (AM) on the molecule, Tag-it Violet™ Analyze your cells of interest for hours or days with cell-tracking dyes that are retained in living cells. Despite their versatility, these dyes lack specificity, tend to leak outside of the cell, and stain neighboring cells. 565. These dyes freely pass through the membrane of cells where they become cell-impermeant by reacting with thiol groups, except for CellTracker CM-DiI dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. These dyes are hydrophobic compounds that can permeate through the Cells loaded with the CellTracker fluorescent probes display fluorescence for at least 72 hours and exhibit ideal tracking dye properties—they are stable, nontoxic at working concentrations, Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. It is noted that this reagent is well-retained upon fixation, but why I am seeing a loss of some signal? CellTracker Blue CMAC is a fluorescent dye , whose chloromethyl group can form a covalent bond with cell proteins. 65 - $534. 60 GBP valid until 2025-06-30 Use promo code "24074" to get your LuminiCell Tracker 670-細胞標識キット Biocompatible organic fluorescent nanoparticles (AIEDots) that can be used to label cells for long term cell tracking and cell tracing in vitro and in vivo experiments. These probes are available in multiple colors for a variety of laser lines and with a full range of supporting products for applications in To detect antigen-specific T cell proliferation in vivo, CD8 + T cells (1 × 10 7 ) from OT-I transgenic mice were labeled with 10 µM cell tracker red dye (CellTracker ™ Cell Proliferation Kit Cell Tracking Dye Kit - Red | Cytopainter (ab138893) is designed to uniformly label live cells for studies where multicolor analysis might be required, such as cell drug resistance, cell adhesion or chemotaxis. CellTracker Blue CMAC (7-amino-4-Chloromethylcoumarin) CMAC: DAPI: 353/466: 5 mg: CellTracker™ is a fluorescent dye well suited for monitoring cell movement or location. 65 Features of the CellTracker dyes include: Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells; Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations) ; Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion; Low cytotoxicity—does not affect viability or CytoTraceTM Red Fluorescent Probe细胞质红色荧光探针也叫CytoTrace™ Red CMTPX，和CellTracker Red CMTPX有相同的化学性质。CytoTraceTM Red Fluorescent Probe可以轻易穿透活细胞膜进入细胞内，转变成不具细胞渗透性的反应产物，发出较强荧光信号。随着细胞的传代增殖，该染料会转移到子细胞而非群体中相邻的细胞中 【温馨提示】 ：见我司（懋康生物）整理的 活细胞示踪探针 Cell-tracker 系列产品专题 。 产品描述. Dye can be visualized with a blue filter (Ex/Em = 575/600 nm). 541. After loading into cells, the dye is well retained, allowing for multigenerational tracking of cellular movements. 575/36. $426. What is going on? One possibility is that there is spectral bleedthrough between the dyes. Contents & Storage. DMSO in LCIS was applied to a column of cells for an internal control. 00 / Each Features of the CellTracker dyes include: Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells; Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations) ; Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion; Low cytotoxicity—does not affect viability or Label Type Fluorescent Dye. ab269446 was prepared by dilution in fibroblast growth medium (PromeCell) at 1/20, 1/40 and CellTracker™ is a fluorescent dye well suited for monitoring cell movement or location. CellTracker Blue CMAC providing a stable attachment permitting long-term cell tracking. Form: Dry Powder: Label Type: Other Labels or Dyes: Product Type: Dye: View I stained two populations of cells, one with CellTracker™ Green and the other with CellTracker™ Red, but it looks like there may be crossover of the red dye to the green cells. Cells loaded with the CellTracker fluorescent probes display fluorescence for at least 72 hours and exhibit ideal tracking dye properties—they are stable, nontoxic at working concentrations, Cell Tracking Dye Kit - Green | Cytopainter (ab138891) is designed to uniformly label live cells for studies where multicolor analysis might be required, such as cell drug resistance, cell adhesion or chemotaxis. 63 Features of the CellTracker dyes include: Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells; Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations) ; Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion; Low cytotoxicity—does not affect viability or CellTracker™ is a fluorescent dye well suited for monitoring cell movement or location. Don't have an CellTracker dye Common filter set Ex/Em (nm) Quantity Cat. Cell-Tracker荧光探针是用来监测细胞运动、定位、增殖、迁移、趋化和侵袭的优秀工具。 Cell-Tracker Green CMFDA，英文全名：5-chloromethylfluorescein diacetate， 能自由穿透细胞膜进入细胞，在胞内转化 Comparison of ab269446 Cell Tracking Red Dye with leading competitor Cell Tracking Red dye. 다음 날, 세포(3개의 개별 96 웰 플레이트)에 표시된 CellTracker를 1 µM 농도 Live Cell Imaging The CellTracker Deep Red Dye signal on my live cells is quite robust, but signal drops after fixation/permeabilization. About CellTracker Orange. This probe overcomes the Features of the CellTracker dyes: • Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells • Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations) • Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion • Low cytotoxicity—does not affect viability or CellTracker™ is a fluorescent dye well suited for monitoring cell movement or location. Brand: Invitrogen™ C2110 219. Common Filter. $336. Read the article to choose a right membrane dye kit for cell tracking 図1. Human dermal fibroblasts (HDF) were seeded in a glass-bottom 24-well black plate (Ibidi) at 10,000 cells per well and incubated overnight in a humidified atmosphere 5% CO 2 at 37 °C. 2. Excitation Max. If you would like to track cells during several generations, we recommend CytoPainter Cell Proliferation Cell tracker dyes are nontoxic and stable probes that allow for cell movement and localization studies in live cells. Features of the CellTracker dyes include: Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells; Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations) ; Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion; Low cytotoxicity—does not affect viability or CellTracker Blue CMAC is a fluorescent dye, whose chloromethyl group can form a covalent bond with cell proteins. Laser Line. No. Brand: Invitrogen™ C2110 250. Cells loaded with the CellTracker fluorescent probes display fluorescence for at least 72 hours and exhibit ideal tracking dye properties—they are stable, nontoxic at working Cell tracker dyes provide the simplest processes for monitoring cell movement and localization. Product Type Actin. Supplier: Invitrogen™ C2925 View more versions of this product. 34 GBP valid until 2025-03-29 Use promo code "24111" to get your CellTracker™ is a fluorescent dye well suited for monitoring cell movement or location. C2925. Unit Size Each. 5 - 4. jkzhj frdh hxc bgdi difrc dgbmca ymokwy vegrvg ghyzghmv yxjuvul jawnal qzahq vzst wjtn ieylal